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Issue 46, 2015
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Stochastic binding of Staphylococcus aureus to hydrophobic surfaces

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Abstract

The adhesion of pathogenic bacteria to surfaces is of immense importance for health care applications. Via a combined experimental and computational approach, we studied the initiation of contact in the adhesion process of the pathogenic bacterium Staphylococcus aureus. AFM force spectroscopy with single cell bacterial probes paired with Monte Carlo simulations enabled an unprecedented molecular investigation of the contact formation. Our results reveal that bacteria attach to a surface over distances far beyond the range of classical surface forces via stochastic binding of thermally fluctuating cell wall proteins. Thereby, the bacteria are pulled into close contact with the surface as consecutive proteins of different stiffnesses attach. This mechanism greatly enhances the attachment capability of S. aureus. It, however, can be manipulated by enzymatically/chemically modifying the cell wall proteins to block their consecutive binding. Our study furthermore reveals that fluctuations in protein density and structure are much more relevant than the exact form of the binding potential.

Graphical abstract: Stochastic binding of Staphylococcus aureus to hydrophobic surfaces

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Supplementary files

Article information


Submitted
22 Apr 2015
Accepted
27 Jul 2015
First published
21 Aug 2015

Soft Matter, 2015,11, 8913-8919
Article type
Paper
Author version available

Stochastic binding of Staphylococcus aureus to hydrophobic surfaces

N. Thewes, A. Thewes, P. Loskill, H. Peisker, M. Bischoff, M. Herrmann, L. Santen and K. Jacobs, Soft Matter, 2015, 11, 8913 DOI: 10.1039/C5SM00963D

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