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Issue 11, 2015
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A “turn on” fluorescent probe for heparin and its oversulfated chondroitin sulfate contaminant

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Abstract

Designing “turn on” fluorescent probes for heparin (Hep), a widely used anticoagulant in clinics, is of great importance but remains challenging. By introducing a Hep specific binding peptide AG73 to a typical aggregation induced emission (AIE) fluorogen, tetraphenylethene (TPE), a sensitive and selective “turn on” fluorescent probe named TPE-1 for Hep was developed. TPE-1 was able to detect Hep in a wide pH range of 3–10 without obvious interference from tested anions and biomolecules, especially Hep analogues known as chondroitin sulfate (Chs) and hyaluronic acid (HA). The detection limit of Hep sensing was 3.8 ng mL−1, which was far below the clinically demanded concentration of Hep. The probe was applicable to both unfractionated Hep and low molecular weight Hep, the two main heparin products clinically used. Besides, the fluorescence of Hep bound TPE-1 can be turned off via sequential treatment with heparinases. Importantly, this phenomenon allows us to develop an enzyme assisted strategy for “turn on” sensing of oversulfated chondroitin sulfate (OSCS) with a detection limit of 0.001% (w%), which is the main contaminant in Hep and may cause severe adverse reactions including death.

Graphical abstract: A “turn on” fluorescent probe for heparin and its oversulfated chondroitin sulfate contaminant

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Publication details

The article was received on 07 May 2015, accepted on 23 Jul 2015 and first published on 23 Jul 2015


Article type: Edge Article
DOI: 10.1039/C5SC01675D
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Chem. Sci., 2015,6, 6361-6366
  • Open access: Creative Commons BY-NC license
    All publication charges for this article have been paid for by the Royal Society of Chemistry

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    A “turn on” fluorescent probe for heparin and its oversulfated chondroitin sulfate contaminant

    Y. Ding, L. Shi and H. Wei, Chem. Sci., 2015, 6, 6361
    DOI: 10.1039/C5SC01675D

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

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