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Issue 69, 2015
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A new methodology for optical biosensing with drop-casting fabrication of sensor chips and irradiation/detection of a single laser beam

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Abstract

We propose a new methodology for optical biosensing with drop-casting fabrication of sensor chips and irradiation/detection of a single laser beam. Glucose sensor chips were fabricated by simply depositing a solution, including o-phenylenediamine (oPD), glucose oxidase (GOD), and horseradish peroxidase (HRP), on a glass substrate. A sample solution was dropped on the sensor chip and the concentration was detected by measuring the reflection intensity of the laser beam focused on the chip. A surface deformation induced by the laser irradiation on the chip was observed by electron microscopy, demonstrating that this sensor chip converts the enzyme reactions into morphological changes using the assistance of the laser beam. The mechanism of the laser-induced surface deformation was investigated by testing the effect of sodium azide and fluorescence spectroscopy.

Graphical abstract: A new methodology for optical biosensing with drop-casting fabrication of sensor chips and irradiation/detection of a single laser beam

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Publication details

The article was received on 03 Mar 2015, accepted on 22 Jun 2015 and first published on 23 Jun 2015


Article type: Paper
DOI: 10.1039/C5RA03754A
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RSC Adv., 2015,5, 56423-56429

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    A new methodology for optical biosensing with drop-casting fabrication of sensor chips and irradiation/detection of a single laser beam

    H. Yoshikawa, S. Imura and E. Tamiya, RSC Adv., 2015, 5, 56423
    DOI: 10.1039/C5RA03754A

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