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Issue 30, 2015
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Microbial cell lysis and nucleic acid extraction via nanofluidic channel

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Abstract

This paper presents a microfluidic device with a nano-channel prepared by focused ion beam (FIB) milling for microbial cell lysis and nucleic acid extraction. The device prepared in a quartz slide combines two microchannels connected with one nanochannel in the middle with dimensions of 100 nm (width) and 250 nm (depth) and 1 μm (length). The capturing of a single or a few cells and their lysis for extraction of DNA in nanochannel were demonstrated. The purity and quantity of the extracted genetic material was tested using a UV spectrometer. The results revealed that the extraction methodology was successful and may be well-suited to integration in analytical lab-on-a-chip devices.

Graphical abstract: Microbial cell lysis and nucleic acid extraction via nanofluidic channel

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Article information


Submitted
26 Jan 2015
Accepted
25 Feb 2015
First published
04 Mar 2015

This article is Open Access

RSC Adv., 2015,5, 23886-23891
Article type
Paper
Author version available

Microbial cell lysis and nucleic acid extraction via nanofluidic channel

K. Kant, J. Yoo, S. Amos, M. Erkelens, C. Priest, J. G. Shapter and D. Losic, RSC Adv., 2015, 5, 23886
DOI: 10.1039/C5RA01336D

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    [Original citation] - Published by The Royal Society of Chemistry.

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