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Issue 10, 2015
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Aqueous RAFT synthesis of block and statistical copolymers of 2-(α-D-mannopyranosyloxy)ethyl methacrylate with 2-(N,N-dimethylamino)ethyl methacrylate and their application for nonviral gene delivery

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Abstract

Block copolymers composed of 2-(α-D-mannopyranosyloxy)ethyl methacrylate (ManEMA) and 2-(N,N-dimethylamino)ethyl methacrylate (DMAEMA) were synthesized by aqueous RAFT polymerization. The number-average degree of polymerization (DPn) of ManEMA segments was constant at 33, and the DPn of DMAEMA segments varied from 98 to 241. Statistical copolymers with a similar composition were also prepared via aqueous RAFT polymerization. The mannose-presenting nature was evaluated by a turbidimetric assay using Concanavalin A. The clustering rate of statistical copolymers was faster than those of the corresponding block copolymers. By contrast, no significant differences between block and statistical copolymers were found in their DNA-condensing ability as evaluated using gel shift assays and in their cytotoxicity in the transfection of plasmid DNA (pEGFP-N1) to HeLa cells. However, the overall transfection efficiency significantly depended on the monomer distribution. Statistical copolymers showed an overall transfection efficiency comparable to those of poly(DMAEMA·HCl)s, but block copolymers showed no detectable transfection under the same conditions.

Graphical abstract: Aqueous RAFT synthesis of block and statistical copolymers of 2-(α-d-mannopyranosyloxy)ethyl methacrylate with 2-(N,N-dimethylamino)ethyl methacrylate and their application for nonviral gene delivery

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Publication details

The article was received on 30 Nov 2014, accepted on 18 Dec 2014 and first published on 19 Dec 2014


Article type: Paper
DOI: 10.1039/C4PY01652A
Polym. Chem., 2015,6, 1793-1804

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    Aqueous RAFT synthesis of block and statistical copolymers of 2-(α-D-mannopyranosyloxy)ethyl methacrylate with 2-(N,N-dimethylamino)ethyl methacrylate and their application for nonviral gene delivery

    M. Obata, T. Kobori, S. Hirohara and M. Tanihara, Polym. Chem., 2015, 6, 1793
    DOI: 10.1039/C4PY01652A

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