Jump to main content
Jump to site search

Issue 13, 2015
Previous Article Next Article

Real-time monitoring of enzyme-free strand displacement cascades by colorimetric assays

Author affiliations

Abstract

The enzyme-free toehold-mediated strand displacement reaction has shown potential for building programmable DNA circuits, biosensors, molecular machines and chemical reaction networks. Here we report a simple colorimetric method using gold nanoparticles as signal generators for the real-time detection of the product of the strand displacement cascade. During the process the assembled gold nanoparticles can be separated, resulting in a color change of the solution. This assay can also be applied in complex mixtures, fetal bovine serum, and to detect single-base mismatches. These results suggest that this method could be of general utility to monitor more complex enzyme-free strand displacement reaction-based programmable systems or for further low-cost diagnostic applications.

Graphical abstract: Real-time monitoring of enzyme-free strand displacement cascades by colorimetric assays

Back to tab navigation

Supplementary files

Publication details

The article was received on 30 Jan 2015, accepted on 16 Feb 2015 and first published on 19 Feb 2015


Article type: Paper
DOI: 10.1039/C5NR00697J
Author version
available:
Download author version (PDF)
Nanoscale, 2015,7, 5719-5725

  •   Request permissions

    Real-time monitoring of enzyme-free strand displacement cascades by colorimetric assays

    R. Duan, B. Wang, F. Hong, T. Zhang, Y. Jia, J. Huang, A. Hakeem, N. Liu, X. Lou and F. Xia, Nanoscale, 2015, 7, 5719
    DOI: 10.1039/C5NR00697J

Search articles by author

Spotlight

Advertisements