Jump to main content
Jump to site search

Issue 19, 2015
Previous Article Next Article

Cell-sized condensed collagen microparticles for preparing microengineered composite spheroids of primary hepatocytes

Author affiliations

Abstract

The reconstitution of extracellular matrix (ECM) components in three-dimensional (3D) cell culture environments with microscale precision is a challenging issue. ECM microparticles would potentially be useful as solid particulate scaffolds that can be incorporated into 3D cellular constructs, but technologies for transforming ECM proteins into cell-sized stable particles are currently lacking. Here, we describe new processes to produce highly condensed collagen microparticles by means of droplet microfluidics or membrane emulsification. Droplets of an aqueous solution of type I collagen were formed in a continuous phase of polar organic solvent followed by rapid dissolution of water molecules into the continuous phase because the droplets were in a non-equilibrium state. We obtained highly unique, disc-shaped condensed collagen microparticles with a final collagen concentration above 10% and examined factors affecting particle size and morphology. After testing the cell-adhesion properties on the collagen microparticles, composite multicellular spheroids comprising the particles and primary rat hepatocytes were formed using microfabricated hydrogel chambers. We found that the ratio of the cells and particles is critical in terms of improvement of hepatic functions in the composite spheroids. The presented methodology for incorporating particulate-form ECM components in multicellular spheroids would be advantageous because of the biochemical similarity with the microenvironments in vivo.

Graphical abstract: Cell-sized condensed collagen microparticles for preparing microengineered composite spheroids of primary hepatocytes

Back to tab navigation

Publication details

The article was received on 06 Jul 2015, accepted on 12 Aug 2015 and first published on 12 Aug 2015


Article type: Paper
DOI: 10.1039/C5LC00785B
Author version
available:
Download author version (PDF)
Lab Chip, 2015,15, 3941-3951

  •   Request permissions

    Cell-sized condensed collagen microparticles for preparing microengineered composite spheroids of primary hepatocytes

    M. Yamada, A. Hori, S. Sugaya, Y. Yajima, R. Utoh, M. Yamato and M. Seki, Lab Chip, 2015, 15, 3941
    DOI: 10.1039/C5LC00785B

Search articles by author

Spotlight

Advertisements