Issue 11, 2015

Exosome isolation: a microfluidic road-map


Exosomes, first isolated 30 years ago, are nanoscale vesicles shed by most types of cells. The nucleic acid rich content of these nanoparticles, floating in virtually all bodily fluids, has great potential for non-invasive molecular diagnostics and may represent a novel therapeutic delivery system. However, current isolation techniques such as ultracentrifugation are not convenient and do not result in high purity isolation. This represents an interesting challenge for microfluidic technologies, from a cost-effective perspective as well as for enhanced purity capabilities, and point-of-care acquisition and diagnosis. In this frontier review, we present the current challenges, comment the first microfluidic advances in this new field and propose a roadmap for future developments. This review enables biologists and clinicians familiar with exosome enrichment to assess the performance of novel microfluidic devices and, equally, enables microfluidic engineers to educate themselves about this new class of promising biomarker-rich particles and the challenges arising from their clinical use.

Graphical abstract: Exosome isolation: a microfluidic road-map

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Article information

Article type
28 Feb 2015
28 Apr 2015
First published
28 Apr 2015
This article is Open Access
Creative Commons BY license

Lab Chip, 2015,15, 2388-2394

Exosome isolation: a microfluidic road-map

A. Liga, A. D. B. Vliegenthart, W. Oosthuyzen, J. W. Dear and M. Kersaudy-Kerhoas, Lab Chip, 2015, 15, 2388 DOI: 10.1039/C5LC00240K

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