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Issue 2, 2015
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A microfluidic device and automatic counting system for the study of C. elegans reproductive aging

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Abstract

The nematode Caenorhabditis elegans (C. elegans) is an excellent model to study reproductive aging because of its short life span, its cessation of reproduction in mid-adulthood, and the strong conservation of pathways that regulate longevity. During its lifetime, a wild-type C. elegans hermaphrodite usually lays about 200–300 self-fertilized hatchable eggs, which mainly occurs in the first three to five days of adulthood. Here, we report the development of a microfluidic assay and a real-time, automatic progeny counting system that records progeny counting information from many individual C. elegans hermaphrodites. This system offers many advantages compared to conventional plate assays. The flow of non-proliferating bacteria not only feeds the worms but also flushes the just-hatched young progeny through a filter that separates mothers from their offspring. The progeny that are flushed out of the chamber are detected and recorded using a novel algorithm. In our current design, one device contains as many as 16 individual chambers. Here we show examples of real-time progeny production information from wild-type (N2) and daf-2 (insulin receptor) mutants. We believe that this system has the potential to become a powerful, high time-resolution tool to study the detailed reproduction of C. elegans.

Graphical abstract: A microfluidic device and automatic counting system for the study of C. elegans reproductive aging

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Supplementary files

Article information


Submitted
02 Sep 2014
Accepted
06 Nov 2014
First published
10 Nov 2014

Lab Chip, 2015,15, 524-531
Article type
Paper
Author version available

A microfluidic device and automatic counting system for the study of C. elegans reproductive aging

S. Li, H. A. Stone and C. T. Murphy, Lab Chip, 2015, 15, 524
DOI: 10.1039/C4LC01028K

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