Issue 2, 2015

An integrated centrifugo-opto-microfluidic platform for arraying, analysis, identification and manipulation of individual cells

Abstract

In this work we present a centrifugal microfluidic system enabling highly efficient collective trapping and alignment of particles such as microbeads and cells, their multi-colour fluorescent detection and subsequent manipulation by optical tweezers. We demonstrate array-based capture and imaging followed by “cherry-picking” of individual particles, first for fluorescently labelled polystyrene (PS) beads and then for cells. Different cell lines are discriminated based on intracellular as well as surface-based markers.

Graphical abstract: An integrated centrifugo-opto-microfluidic platform for arraying, analysis, identification and manipulation of individual cells

Supplementary files

Article information

Article type
Technical Innovation
Submitted
28 Aug 2014
Accepted
17 Oct 2014
First published
12 Nov 2014

Lab Chip, 2015,15, 378-381

An integrated centrifugo-opto-microfluidic platform for arraying, analysis, identification and manipulation of individual cells

R. Burger, D. Kurzbuch, R. Gorkin, G. Kijanka, M. Glynn, C. McDonagh and J. Ducrée, Lab Chip, 2015, 15, 378 DOI: 10.1039/C4LC01002G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements