Issue 1, 2015

An integrated microspectrometer for localised multiplexing measurements


We describe the development of an integrated lensed Arrayed Waveguide Grating (AWG) microspectrometer for localized multiplexing fluorescence measurements. The device, which has a footprint that is only 1 mm wide and 1 cm long, is capable of spectroscopic measurements on chip. Multiple fluorescence signals were measured simultaneously based upon simple intensity readouts from a CCD camera. We also demonstrate the integration of the AWG spectrometer with a microfluidic platform using a lensing function to confine the beam shape for focused illumination. This capability enhances signal collection, gives better spatial resolution, and provides a route for the analysis of small volume samples (e.g. cells) in flow. To show these capabilities we developed a novel “bead–AWG” platform with which we demonstrate localized multiplexed fluorescence detection either simultaneously or successively. Such an integrated system provides the basis for a portable system capable of optical detection of multi-wavelength fluorescence from a single defined location.

Graphical abstract: An integrated microspectrometer for localised multiplexing measurements

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Article information

Article type
14 Aug 2014
15 Oct 2014
First published
15 Oct 2014
This article is Open Access
Creative Commons BY license

Lab Chip, 2015,15, 283-289

Author version available

An integrated microspectrometer for localised multiplexing measurements

Z. Hu, A. Glidle, C. Ironside, J. M. Cooper and H. Yin, Lab Chip, 2015, 15, 283 DOI: 10.1039/C4LC00952E

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