Fast ion chromatography-ICP-QQQ for arsenic speciation

Abstract

Two methods for the fast separation of arsenic species are presented. The general approach is to modify existing methodology utilizing carbonate eluents for a small particle size, short column length Hamilton PRP-X100 column which is interfaced with the Agilent 8800 ICP-QQQ using oxygen as reaction gas and detection of AsO at m/z 91. Using H₂O₂ in the extractant to oxidize As(III) to As(V) it is possible to separate arsenobetaine from DMA, MMA and As(V) in 1.5 minutes. Such a method may be useful where a measure of total inorganic As is sufficient, for example for regulatory compliance in food or beverage testing. It is possible to separate six As species. i.e. the four above and arsenocholine and As(III) in 4.5 minutes using a gradient separation. Such a method could be useful for analysis of urinary arsenic species. Coupling with the high sensitivity of ICP-QQQ yields equivalent or better detection limits than conventional methods with run times up to 5 times faster, which is a significant benefit for sample throughput and method development.