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Issue 2, 2015
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Isolation and identification of ingredients inducing cancer cell death from the seeds of Alpinia galanga, a Chinese spice

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Abstract

This study was carried out to isolate ingredients from the seeds of a Chinese spice (Alpinia galangal) and to evaluate their cytotoxic activity on cancer cell lines. Isolation and purification of the phytochemical constituents were conducted using silica gel, Sephadex LH-20 and ODS columns. After extraction using 95% ethanol, the total extracts were re-extracted, resulting in petroleum ether (PE), ethyl acetate (EA) and water fractions, respectively. Activity tests showed that the EA fraction exhibited obvious (p < 0.05) protective effects on H2O2 damaged PC-12 cells at 20 μg mL−1, and showed much higher (p < 0.05) cytotoxic activity on cancer cell lines than other fractions. Five compounds, 1′-S-1′-acetoxyeugenol acetate (I), 1′-S-1′-acetoxychavicol acetate (II), 2-propenal, 3-[4-(acetyloxy)-3-methoxyphenyl] (III), isocoronarin D (IV) and caryolane-1, 9β-diol (V), were obtained from the EA fraction and identified by HPLC, UV, MS, and NMR spectroscopic analyses. Compounds III and V were isolated from A. galangal for the first time. Moreover, compounds I, II, IV and V were the main active ingredients for inducing death of the tested cancer cells, and their IC50 values ranged from 60 to 90 μg mL−1, indicating that these compounds possessed a wide anti-cancer capability. Therefore, A. galangal seeds could be a potential source of healthy food for tumor prevention.

Graphical abstract: Isolation and identification of ingredients inducing cancer cell death from the seeds of Alpinia galanga, a Chinese spice

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Publication details

The article was received on 06 Aug 2014, accepted on 24 Nov 2014 and first published on 25 Nov 2014


Article type: Paper
DOI: 10.1039/C4FO00709C
Food Funct., 2015,6, 431-443

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    Isolation and identification of ingredients inducing cancer cell death from the seeds of Alpinia galanga, a Chinese spice

    Q. Zeng, C. Lu, X. Zhang and J. Jiang, Food Funct., 2015, 6, 431
    DOI: 10.1039/C4FO00709C

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