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Volume 177, 2015
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Resolution enhancement through microscopic spatiotemporal control

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Abstract

Operating at biologically benign conditions, multi-photon fluorescence imaging microscopy has benefitted immensely from recent developments in microscopic resolution enhancement. Fluorescence microscopy continues to be the best choice for experiments on live specimens, however, multi-photon fluorescence imaging often suffers from overlapping fluorescence of typical dyes used in microscopy, limiting its scope. This limitation has been the focus of our research where we show that by making simple modifications to the laser pulse structure, it is possible to resolve these overlapping fluorescence complications. Specifically, by using pairs of femtosecond pulses with variable delay in place of single pulse excitation, we show controlled fluorescence excitation or suppression of one of the fluorophores over the other through wave-packet interferometry. Such an effect prevails even after the fluorophore coherence timescale, which effectively results in a higher spatial resolution. Here we extend the effect of our pulse-pair technique to microscopic axial resolution experiments and show that such pairs of pulses can also ‘enhance’ axial resolution.

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Publication details

The article was received on 15 Sep 2014, accepted on 24 Nov 2014 and first published on 27 Jan 2015


Article type: Paper
DOI: 10.1039/C4FD00177J
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Faraday Discuss., 2015,177, 203-212

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    Resolution enhancement through microscopic spatiotemporal control

    D. Goswami, D. Das and S. Nath Bandyopadhyay, Faraday Discuss., 2015, 177, 203
    DOI: 10.1039/C4FD00177J

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