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Issue 41, 2015
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A peptide-based fluorescent chemosensor for measuring cadmium ions in aqueous solutions and live cells

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Abstract

A novel peptide fluorescent chemosensor (H2L) with a lysine backbone and both –NH2 sites conjugated with cysteine and dansyl groups has been designed and synthesized by solid phase peptide synthesis with Fmoc chemistry. This chemosensor is a promising analytical tool for detecting Cd2+ based on the photo-induced electron transfer (PET) effect by turn-on response in 100% aqueous solutions. As designed, H2L exhibits excellent cell permeation and low biotoxicity as well as displaying relatively high selectivity and sensitivity. The chemosensor penetrated live HeLa cells and detected intracellular Cd2+ by turn-on response. The binding stoichiometry and affinity, interference test, pH sensitivity, fluorescence quantum yield, quantum mechanical calculations, lifetimes, and cytotoxicity of the chemosensor H2L to Cd2+ were also investigated. Moreover, H2L exhibits low biotoxicity with a limit of detection (LOD) for Cd2+ of about 52 nM, implying that H2L can be used as a highly selective and sensitive peptide fluorescent chemosensor in biological systems.

Graphical abstract: A peptide-based fluorescent chemosensor for measuring cadmium ions in aqueous solutions and live cells

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Publication details

The article was received on 15 Aug 2015, accepted on 10 Sep 2015 and first published on 16 Sep 2015


Article type: Paper
DOI: 10.1039/C5DT03156G
Dalton Trans., 2015,44, 18057-18064

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    A peptide-based fluorescent chemosensor for measuring cadmium ions in aqueous solutions and live cells

    P. Wang, J. Wu, L. Liu, P. Zhou, Y. Ge, D. Liu, W. Liu and Y. Tang, Dalton Trans., 2015, 44, 18057
    DOI: 10.1039/C5DT03156G

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