Jump to main content
Jump to site search

Issue 37, 2015
Previous Article Next Article

Unexpected DNA binding properties with correlated downstream biological applications in mono vs. bis-1,8-naphthalimide Ru(ii)-polypyridyl conjugates

Author affiliations

Abstract

The synthesis, spectroscopic characterisation and biological evaluation of mono- and bis-1,8-naphthalimide-conjugated ruthenium(II)-polypyridyl complexes is presented. Spectroscopic DNA titrations, together with denaturation studies, show strong binding of both species to DNA through the naphthalimide arms. Linear and circular dichroism (LD and CD) spectroscopy reveal close association of the Ru(bpy)32+ core with DNA in the case of the mono-naphthalamide complex, [Ru(bpy)2(bpy-NAP)]2+. Significantly, binding by the second naphthalimide arm in the [Ru(bpy)2(bpy-NAP2)]2+ complex is found to displace the Ru(bpy)32+ centre from the DNA backbone. This ‘negative allosteric effect’ is found to have a dramatic influence on the photoinduced damage of plasmid DNA, and the viability of HeLa cancer cells upon photoactivation. Overall the study clearly maps and correlates the relationship between molecular structure, in vitro binding and activity, and in cellulo function.

Graphical abstract: Unexpected DNA binding properties with correlated downstream biological applications in mono vs. bis-1,8-naphthalimide Ru(ii)-polypyridyl conjugates

Back to tab navigation

Supplementary files

Article information


Submitted
26 Jan 2015
Accepted
05 Aug 2015
First published
05 Aug 2015

Dalton Trans., 2015,44, 16332-16344
Article type
Paper
Author version available

Unexpected DNA binding properties with correlated downstream biological applications in mono vs. bis-1,8-naphthalimide Ru(II)-polypyridyl conjugates

G. J. Ryan, F. E. Poynton, R. B. P. Elmes, M. Erby, D. C. Williams, S. J. Quinn and T. Gunnlaugsson, Dalton Trans., 2015, 44, 16332
DOI: 10.1039/C5DT00360A

Social activity

Search articles by author

Spotlight

Advertisements