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Issue 33, 2015
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Enhanced-fluorescence correlation spectroscopy at micro-molar dye concentration around a single gold nanorod

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Abstract

Fluorescence correlation spectroscopy (FCS) is a standard tool for studying diffusion of molecules in solution, but is limited to low analyte concentrations, in the range between 10 pM and 1 nM. Such concentration limitations can be overcome by using a plasmonic nanoantenna which confines the electric field of excitation light into a tiny volume near its surface and thereby reduces the effective excitation volume by several orders of magnitude. Here we demonstrate successful FCS measurements on a 1 μM solution of crystal violet (CV) dye in glycerol using a gold nanorod antenna. Our correlation analysis yields two components: (i) a slow component with correlation time of about 100 ms, which is attributed to sticking and bleaching of the dye, and (ii) a fast component of about 1 ms, which could arise from dye diffusion through the near-field of the nanorod and/or from blinking due to intersystem crossing or photochemistry.

Graphical abstract: Enhanced-fluorescence correlation spectroscopy at micro-molar dye concentration around a single gold nanorod

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Supplementary files

Article information


Submitted
11 Jul 2014
Accepted
29 Aug 2014
First published
01 Sep 2014

Phys. Chem. Chem. Phys., 2015,17, 21127-21132
Article type
Paper
Author version available

Enhanced-fluorescence correlation spectroscopy at micro-molar dye concentration around a single gold nanorod

S. Khatua, H. Yuan and M. Orrit, Phys. Chem. Chem. Phys., 2015, 17, 21127
DOI: 10.1039/C4CP03057E

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