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Issue 97, 2015
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Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation

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Abstract

Using a recently discovered peptide ligase, butelase 1, we developed a novel method to access protein thioesters in good yield. We successfully combined it with native chemical ligation and sortase-mediated ligation in tandem for protein C-terminal labeling and dual-terminal labeling to exploit the orthogonality of these three ligation methods.

Graphical abstract: Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation

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Publication details

The article was received on 28 Aug 2015, accepted on 06 Oct 2015 and first published on 06 Oct 2015


Article type: Communication
DOI: 10.1039/C5CC07227A
Chem. Commun., 2015,51, 17289-17292

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    Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation

    Y. Cao, G. K. T. Nguyen, J. P. Tam and C. Liu, Chem. Commun., 2015, 51, 17289
    DOI: 10.1039/C5CC07227A

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