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Issue 63, 2015
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Enhancing the efficiency of sortase–mediated ligations through nickel–peptide complex formation

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Abstract

A modified sortase A recognition motif containing a masked Ni2+-binding peptide was employed to boost the efficiency of sortase-catalyzed ligation reactions. Deactivation of the Ni2+-binding peptide using a Ni2+ additive improved reaction performance at low to equimolar ratios of the glycine amine nucleophile and sortase substrate. The success of this approach was demonstrated with both peptide and protein substrates.

Graphical abstract: Enhancing the efficiency of sortase–mediated ligations through nickel–peptide complex formation

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Supplementary files

Article information


Submitted
05 Jun 2015
Accepted
02 Jul 2015
First published
02 Jul 2015

Chem. Commun., 2015,51, 12548-12551
Article type
Communication
Author version available

Enhancing the efficiency of sortase–mediated ligations through nickel–peptide complex formation

R. David Row, T. J. Roark, M. C. Philip, L. L. Perkins and J. M. Antos, Chem. Commun., 2015, 51, 12548
DOI: 10.1039/C5CC04657B

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