Jump to main content
Jump to site search

Issue 81, 2015
Previous Article Next Article

Nano-mole scale sequential signal assignment by 1H-detected protein solid-state NMR

Author affiliations

Abstract

We present a 3D 1H-detected solid-state NMR (SSNMR) approach for main-chain signal assignments of 10–100 nmol of fully protonated proteins using ultra-fast magic-angle spinning (MAS) at ∼80 kHz by a novel spectral-editing method, which permits drastic spectral simplification. The approach offers ∼110 fold time saving over a traditional 3D 13C-detected SSNMR approach.

Graphical abstract: Nano-mole scale sequential signal assignment by 1H-detected protein solid-state NMR

Back to tab navigation

Supplementary files

Publication details

The article was received on 04 Jun 2015, accepted on 14 Aug 2015 and first published on 28 Aug 2015


Article type: Communication
DOI: 10.1039/C5CC04618A
Author version
available:
Download author version (PDF)
Chem. Commun., 2015,51, 15055-15058

  •   Request permissions

    Nano-mole scale sequential signal assignment by 1H-detected protein solid-state NMR

    S. Wang, S. Parthasarathy, Y. Xiao, Y. Nishiyama, F. Long, I. Matsuda, Y. Endo, T. Nemoto, K. Yamauchi, T. Asakura, M. Takeda, T. Terauchi, M. Kainosho and Y. Ishii, Chem. Commun., 2015, 51, 15055
    DOI: 10.1039/C5CC04618A

Search articles by author

Spotlight

Advertisements