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Issue 23, 2015
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Polymerase synthesis of DNA labelled with benzylidene cyanoacetamide-based fluorescent molecular rotors: fluorescent light-up probes for DNA-binding proteins

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Abstract

Viscosity-sensitive fluorophores, fluorescent molecular rotors based on aminobenzylidene–cyanoacetamide moiety, were tethered to 2′-deoxycytidine triphosphate via a propargylamine linker and incorporated into DNA by polymerases in primer extension, nicking enzyme amplification or PCR. DNA probes incorporating modified nucleosides show a light-up response upon binding to a protein.

Graphical abstract: Polymerase synthesis of DNA labelled with benzylidene cyanoacetamide-based fluorescent molecular rotors: fluorescent light-up probes for DNA-binding proteins

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Supplementary files

Article information


Submitted
19 Jan 2015
Accepted
10 Feb 2015
First published
11 Feb 2015

This article is Open Access

Chem. Commun., 2015,51, 4880-4882
Article type
Communication

Polymerase synthesis of DNA labelled with benzylidene cyanoacetamide-based fluorescent molecular rotors: fluorescent light-up probes for DNA-binding proteins

D. Dziuba, R. Pohl and M. Hocek, Chem. Commun., 2015, 51, 4880
DOI: 10.1039/C5CC00530B

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