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Issue 22, 2015
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Improved one-pot multienzyme (OPME) systems for synthesizing UDP-uronic acids and glucuronides

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Abstract

Arabidopsis thaliana glucuronokinase (AtGlcAK) was cloned and shown to be able to use various uronic acids as substrates to produce the corresponding uronic acid-1-phosphates. AtGlcAK or Bifidobacterium infantis galactokinase (BiGalK) was used with a UDP-sugar pyrophosphorylase, an inorganic pyrophosphatase, with or without a glycosyltransferase for highly efficient synthesis of UDP-uronic acids and glucuronides. These improved cost-effective one-pot multienzyme (OPME) systems avoid the use of nicotinamide adenine dinucleotide (NAD+)-cofactor in dehydrogenase-dependent UDP-glucuronic acid production processes and can be broadly applied for synthesizing various glucuronic acid-containing molecules.

Graphical abstract: Improved one-pot multienzyme (OPME) systems for synthesizing UDP-uronic acids and glucuronides

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Supplementary files

Article information


Submitted
24 Dec 2014
Accepted
09 Feb 2015
First published
09 Feb 2015

Chem. Commun., 2015,51, 4595-4598
Article type
Communication
Author version available

Improved one-pot multienzyme (OPME) systems for synthesizing UDP-uronic acids and glucuronides

M. M. Muthana, J. Qu, M. Xue, T. Klyuchnik, A. Siu, Y. Li, L. Zhang, H. Yu, L. Li, P. G. Wang and X. Chen, Chem. Commun., 2015, 51, 4595
DOI: 10.1039/C4CC10306H

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