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Issue 14, 2015
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A synthetic biology approach for the transformation of l-α-amino acids to the corresponding enantiopure (R)- or (S)-α-hydroxy acids

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Abstract

Combinatorial assembly and variation of promoters on a single expression plasmid allowed the balance of the catalytic steps of a three enzyme (L-AAD, HIC, FDH) cascade in E. coli. The designer cell catalyst quantitatively transformed L-amino acids to the corresponding optically pure (R)- and (S)-α-hydroxy acids at up to 200 mM substrate concentration.

Graphical abstract: A synthetic biology approach for the transformation of l-α-amino acids to the corresponding enantiopure (R)- or (S)-α-hydroxy acids

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Supplementary files

Article information


Submitted
20 Oct 2014
Accepted
02 Dec 2014
First published
09 Jan 2015

Chem. Commun., 2015,51, 2828-2831
Article type
Communication
Author version available

A synthetic biology approach for the transformation of L-α-amino acids to the corresponding enantiopure (R)- or (S)-α-hydroxy acids

G. Gourinchas, E. Busto, M. Killinger, N. Richter, B. Wiltschi and W. Kroutil, Chem. Commun., 2015, 51, 2828
DOI: 10.1039/C4CC08286A

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