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Issue 1, 2015
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Label-free, isothermal and ultrasensitive electrochemical detection of DNA and DNA 3′-phosphatase using a cascade enzymatic cleavage strategy

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Abstract

Label-free and ultrasensitive electrochemical assays of target DNA and T4 polynucleotide kinase phosphatase (PNKP) were developed, which took full advantage of three enzymes to realize the signal readout and amplification. It can ultimately achieve the low detection limits of 10 fM and 1 mU mL−1 for target DNA and PNKP, respectively.

Graphical abstract: Label-free, isothermal and ultrasensitive electrochemical detection of DNA and DNA 3′-phosphatase using a cascade enzymatic cleavage strategy

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Publication details

The article was received on 14 Oct 2014, accepted on 31 Oct 2014 and first published on 31 Oct 2014


Article type: Communication
DOI: 10.1039/C4CC08140D
Chem. Commun., 2015,51, 176-179

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    Label-free, isothermal and ultrasensitive electrochemical detection of DNA and DNA 3′-phosphatase using a cascade enzymatic cleavage strategy

    S. Liu, T. Liu and L. Wang, Chem. Commun., 2015, 51, 176
    DOI: 10.1039/C4CC08140D

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