Issue 3, 2015
From the journal:

Chemical Communications

Combining in-cell NMR and X-ray fluorescence microscopy to reveal the intracellular maturation states of human superoxide dismutase 1†

E. Luchinat, ORCID logo ab   A. Gianoncelli,c   T. Mello,b   A. Gallib  and  L. Banci*ad  

* Corresponding authors

a Magnetic Resonance Center - CERM, University of Florence, Via Luigi Sacconi 6, Sesto Fiorentino, Florence, Italy
E-mail: banci@cerm.unifi.it

b Department of Biomedical, Clinical and Experimental Sciences, University of Florence, Viale Morgagni 50, Florence, Italy

c Elettra-Sincrotrone Trieste, Area Science Park, Basovizza, Trieste, Italy

d Department of Chemistry, University of Florence, Via della Lastruccia 3, Sesto Fiorentino, Florence, Italy

Abstract

An integrated approach which combines in-cell NMR spectroscopy with optical and X-ray fluorescence microscopy was developed to describe the intracellular maturation state of human Cu,Zn-SOD1. Microscopy data show a correlation between the intracellular levels of SOD1 and the content of zinc, corresponding to zinc binding to SOD1 observed by in-cell NMR.

Graphical abstract: Combining in-cell NMR and X-ray fluorescence microscopy to reveal the intracellular maturation states of human superoxide dismutase 1

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Article information

DOI
https://doi.org/10.1039/C4CC08129C
Article type
Communication
Submitted
14 Oct 2014
Accepted
13 Nov 2014
First published
13 Nov 2014

Chem. Commun., 2015,51, 584-587

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Combining in-cell NMR and X-ray fluorescence microscopy to reveal the intracellular maturation states of human superoxide dismutase 1

E. Luchinat, A. Gianoncelli, T. Mello, A. Galli and L. Banci, Chem. Commun., 2015, 51, 584 DOI: 10.1039/C4CC08129C

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