Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 12, 2015
Previous Article Next Article

Terminus-free siRNA prepared by photo-crosslinking activated via slicing by Ago2

Author affiliations

Abstract

We report the development of photo-crosslinked siRNA strands modified at each terminus with p-cyanostilbene. The siRNA was nuclease resistant and retained RNAi activity. We further studied the activation mechanism of the covalently-crosslinked siRNA. Interestingly Dicer, which is known to generate siRNA with overhanging 3′ ends from the precursor siRNA, did not cleave the crosslinked siRNA at all. Our results suggest that the activation of the crosslinked siRNAs required cleavage by Argonaute2.

Graphical abstract: Terminus-free siRNA prepared by photo-crosslinking activated via slicing by Ago2

Back to tab navigation

Supplementary files

Article information


Submitted
07 Jul 2015
Accepted
23 Oct 2015
First published
03 Nov 2015

Biomater. Sci., 2015,3, 1534-1538
Article type
Communication
Author version available

Terminus-free siRNA prepared by photo-crosslinking activated via slicing by Ago2

Y. Kamiya, K. Iishiba, T. Doi, K. Tsuda, H. Kashida and H. Asanuma, Biomater. Sci., 2015, 3, 1534
DOI: 10.1039/C5BM00231A

Social activity

Search articles by author

Spotlight

Advertisements