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Issue 5, 2015
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Gene expression of ternary complexes through the compaction of nanofiber-polyplexes by mixing with lipofectamine

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Abstract

For the development of an effective nonviral gene vector, ternary complexes were prepared through the compaction of nanofiber-polyplexes. These were formed using pDNA and a head-tail type polycation bearing a multi-arm poly(ethylene glycol) head and a poly(L-lysine) tail, and this strategy was based on the crowding effect of poly(ethylene glycol) in the polyplex. Mixing was carried out using a cationic lipid (lipofectamine), which is a commercially available transfection reagent. Through ternary complex formation, the elongated morphology of nanofiber-polyplexes was found to compact into a spherical shape with an average diameter of ca. 100 nm. Accompanying ternary complex formation, the compaction of the nanofiber-polyplexes can improve cellular uptake and helps the ternary complex to retain its smooth transcription/translation process, which is characteristic of nanofiber-polyplexes. As a result, ternary complexes prepared at an optimal mixing ratio exhibit a high transfection efficiency compared with lipofectamine lipoplexes.

Graphical abstract: Gene expression of ternary complexes through the compaction of nanofiber-polyplexes by mixing with lipofectamine

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Publication details

The article was received on 13 Mar 2015, accepted on 23 Mar 2015 and first published on 02 Apr 2015


Article type: Paper
DOI: 10.1039/C5BM00081E
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Biomater. Sci., 2015,3, 764-770

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    Gene expression of ternary complexes through the compaction of nanofiber-polyplexes by mixing with lipofectamine

    R. Aono, K. Nomura, E. Yuba, A. Harada and K. Kono, Biomater. Sci., 2015, 3, 764
    DOI: 10.1039/C5BM00081E

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