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Issue 2, 2015
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Effective CpG DNA delivery using amphiphilic cycloamylose nanogels

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Abstract

Unmethylated CpG oligodeoxynucleotides induce inflammatory immune responses through cytokine production and have attracted increasing attention as an immunostimulator. However, there remains a challenging issue of the use of ‘native CpG DNA’. In the present study, we prepared cationic nanometer-sized gels (nanogels) consisting of cycloamylose modified with cholesterol and diethylaminoethane to form hydrophobic cross-linking points and to add positively charged groups, respectively. The cationic nanogels and native CpG DNA formed nanometer-sized complexes. Complexes of native CpG DNA with cationic nanogels delivered native CpG DNA to macrophage-like cells and induced cytokine production. In addition, complexes of negative control oligonucleotides with cationic nanogels did not induce cytokine production, and the induction of cytokines using complexes of phosphorothioate-modified CpG with cationic nanogels was lower than that of native CpG DNA. These results suggest that the complex of native CpG DNA with cationic nanogels is a promising strategy for nucleic acid adjuvants.

Graphical abstract: Effective CpG DNA delivery using amphiphilic cycloamylose nanogels

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Supplementary files

Article information


Submitted
12 Aug 2014
Accepted
18 Sep 2014
First published
13 Oct 2014

Biomater. Sci., 2015,3, 256-264
Article type
Paper
Author version available

Effective CpG DNA delivery using amphiphilic cycloamylose nanogels

Y. Tahara, J. Yasuoka, S. Sawada, Y. Sasaki and K. Akiyoshi, Biomater. Sci., 2015, 3, 256
DOI: 10.1039/C4BM00293H

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