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Issue 24, 2015
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A fluorescent probe for the discrimination between Cys and GSH

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Abstract

Fluorescent probe RTP was rationally designed and synthesized to differentially detect cysteine (Cys) and glutathione (GSH) in different channels. RTP was constructed by incorporating a nitrothiophenyl receptor to a rhodamine fluorophore. The replacement of the receptor by the mercapto group in biothiols forms a weakly fluorescent thioether. The amino group in Cys/Hcy further attacks the thioether through aromatic nucleophilic substitution via a five/six-membered ring to form highly fluorescent red-emitting amino-substituted rhodamine. Moreover, RTP has been successfully used for fluorescence imaging of Cys in living Hela cells.

Graphical abstract: A fluorescent probe for the discrimination between Cys and GSH

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Publication details

The article was received on 04 Sep 2015, accepted on 04 Nov 2015 and first published on 06 Nov 2015


Article type: Paper
DOI: 10.1039/C5AY02354H
Anal. Methods, 2015,7, 10371-10375

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    A fluorescent probe for the discrimination between Cys and GSH

    L. Song, Q. Sun, N. Wang, Z. Chen, W. Zhang and J. Qian, Anal. Methods, 2015, 7, 10371
    DOI: 10.1039/C5AY02354H

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