Parallelized label-free detection of protein interactions using a hyper-spectral imaging system
We describe parallelized label-free detection of protein interactions using a hyper-spectral imaging system. Multi-array protein chips were fabricated by immobilizing antibodies on Au-capped nanopillar polymer films, which showed an optical absorption band due to the localised surface plasmon resonance (LSPR) in the absorption spectrum. Parallelized detection of multiple antigen–antibody interactions was demonstrated by processing hyper-spectral images of the multi-array chip before and after the application of antigens. Human immunoglobulin A (IgA) and human C-reactive protein (CRP) were specifically detected, and the signal corresponding to these protein concentrations was obtained, indicating that this technique is promising for use in enhancing the performance of multiple protein assays.