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Issue 12, 2015
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Parallelized label-free detection of protein interactions using a hyper-spectral imaging system

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Abstract

We describe parallelized label-free detection of protein interactions using a hyper-spectral imaging system. Multi-array protein chips were fabricated by immobilizing antibodies on Au-capped nanopillar polymer films, which showed an optical absorption band due to the localised surface plasmon resonance (LSPR) in the absorption spectrum. Parallelized detection of multiple antigen–antibody interactions was demonstrated by processing hyper-spectral images of the multi-array chip before and after the application of antigens. Human immunoglobulin A (IgA) and human C-reactive protein (CRP) were specifically detected, and the signal corresponding to these protein concentrations was obtained, indicating that this technique is promising for use in enhancing the performance of multiple protein assays.

Graphical abstract: Parallelized label-free detection of protein interactions using a hyper-spectral imaging system

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Publication details

The article was received on 19 Mar 2015, accepted on 12 May 2015 and first published on 12 May 2015


Article type: Paper
DOI: 10.1039/C5AY00738K
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Anal. Methods, 2015,7, 5157-5161

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    Parallelized label-free detection of protein interactions using a hyper-spectral imaging system

    H. Yoshikawa, M. Murahashi, M. Saito, S. Jiang, M. Iga and E. Tamiya, Anal. Methods, 2015, 7, 5157
    DOI: 10.1039/C5AY00738K

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