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Issue 1, 2015
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Towards on-site testing of Phytophthora species

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Abstract

Rapid detection and accurate identification of plant pathogens in the field is an ongoing challenge. In this study, we report for the first time on the development of a helicase-dependent isothermal amplification (HDA) in combination with on-chip hybridization for the detection of selected Phytophthora species. The HDA approach allows efficient amplification of the yeast GTP-binding protein (Ypt1) target gene region at one constant temperature in a miniaturized heating device. The assay's specificity was determined by on-chip DNA hybridization and subsequent silver nanoparticle deposition. The silver deposits serve as stable endpoint signals that enable the visual as well as the electrical readout. Our promising results point to the direction of a near future on-site application of the combined techniques for a reliable detection of Phytophthora species.

Graphical abstract: Towards on-site testing of Phytophthora species

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Publication details

The article was received on 26 Sep 2014, accepted on 10 Nov 2014 and first published on 11 Nov 2014


Article type: Paper
DOI: 10.1039/C4AY02287D
Anal. Methods, 2015,7, 211-217
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    Towards on-site testing of Phytophthora species

    L. Schwenkbier, S. Pollok, S. König, M. Urban, S. Werres, D. Cialla-May, K. Weber and J. Popp, Anal. Methods, 2015, 7, 211
    DOI: 10.1039/C4AY02287D

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