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Issue 4, 2015
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Preparative separation of isoflavones in plant extract of Pueraria lobata by high performance counter-current chromatography

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Abstract

Five isoflavones, puerarin, daidzin, daidzein, 3′-hydroxy-puerarin and 3′-methoxy-puerarin were isolated and purified simultaneously from Pueraria lobata for the first time by high performance counter-current chromatography (HPCCC) using a system consisting of hexane–ethyl acetate–n-butanol–ethanol–water (0.5 : 2 : 1 : 0.5 : 3.5, v/v/v/v/v). In total, 155 mg of puerarin, 41 mg of daidzin, 12 mg of 3′-hydroxy-puerarin, 6 mg of 3′-methoxy-puerarin and 106 mg of daidzein were purified from 5.1 g of the ethyl acetate extract of P. lobata; the purities of the five isoflavones as determined by high performance liquid chromatography (HPLC) were 98.77%, 96.53%, 97.59%, 90.21% and 98.36%, respectively. The structures of the five compounds were identified by their retention time and the electrospray ionization multi-stage tandem mass spectrometry (ESI-MSn) in the positive ion mode, and confirmed by 1H-NMR experiments. The characteristic ESI-MS fragmentation patterns of the five compounds are discussed.

Graphical abstract: Preparative separation of isoflavones in plant extract of Pueraria lobata by high performance counter-current chromatography

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Publication details

The article was received on 05 Aug 2014, accepted on 09 Dec 2014 and first published on 11 Dec 2014


Article type: Paper
DOI: 10.1039/C4AY01849D
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Anal. Methods, 2015,7, 1321-1327

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    Preparative separation of isoflavones in plant extract of Pueraria lobata by high performance counter-current chromatography

    B. Li, L. Li, A. Zhao, B. Han, Y. Fan, C. Liu and J. Liu, Anal. Methods, 2015, 7, 1321
    DOI: 10.1039/C4AY01849D

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