Issue 22, 2015

High resolution mass spectrometry imaging of plant tissues: towards a plant metabolite atlas

Abstract

Mass spectrometry (MS) imaging provides spatial and molecular information for a wide range of compounds. This tool can be used to investigate metabolic changes in plant physiology and environmental interactions. A major challenge in our study was to prepare tissue sections that were compatible with high spatial resolution analysis and therefore dedicated sample preparation protocols were established and optimized for the physicochemical properties of all major plant organs. We combined high spatial resolution (5 μm), in order to detect cellular features, and high mass accuracy (<2 ppm root mean square error), for molecular specificity. Mass spectrometry imaging experiments were performed in positive and negative ion mode. Changes in metabolite patterns during plant development were investigated for germination of oilseed rape. The detailed localization of more than 90 compounds allowed assignment to metabolic processes and indicated possible functions in plant tissues. The ‘untargeted’ nature of MS imaging allows the detection of marker compounds for the physiological status, as demonstrated for plant–pathogen interactions. Our images show excellent correlation with optical/histological examination. In contrast to previous MS imaging studies of plants, we present a complete workflow that covers multiple species, such as oilseed rape, wheat seed and rice. In addition, different major plant organs and a wide variety of compound classes were analyzed. Thus, our method could be used to develop a plant metabolite atlas as a reference to investigate systemic and local effects of pathogen infection or environmental stress.

Graphical abstract: High resolution mass spectrometry imaging of plant tissues: towards a plant metabolite atlas

Supplementary files

Article information

Article type
Paper
Submitted
28 May 2015
Accepted
28 Aug 2015
First published
16 Sep 2015
This article is Open Access
Creative Commons BY license

Analyst, 2015,140, 7696-7709

Author version available

High resolution mass spectrometry imaging of plant tissues: towards a plant metabolite atlas

D. R. Bhandari, Q. Wang, W. Friedt, B. Spengler, S. Gottwald and A. Römpp, Analyst, 2015, 140, 7696 DOI: 10.1039/C5AN01065A

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