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Issue 20, 2015
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Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots

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Abstract

Liquid extraction surface analysis (LESA) is a surface sampling technique that allows electrospray mass spectrometry analysis of a wide range of analytes directly from biological substrates. Here, we present LESA mass spectrometry coupled with high field asymmetric waveform ion mobility spectrometry (FAIMS) for the analysis of dried blood spots on filter paper. Incorporation of FAIMS in the workflow enables gas-phase separation of lipid and protein molecular classes, enabling analysis of both haemoglobin and a range of lipids (phosphatidylcholine or phosphatidylethanolamine, and sphingomyelin species) from a single extraction sample. The work has implications for multiplexed clinical assays of multiple analytes.

Graphical abstract: Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots

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Supplementary files

Article information


Submitted
08 May 2015
Accepted
15 Jul 2015
First published
15 Jul 2015

This article is Open Access

Analyst, 2015,140, 6879-6885
Article type
Paper
Author version available

Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots

R. L. Griffiths, A. Dexter, A. J. Creese and H. J. Cooper, Analyst, 2015, 140, 6879
DOI: 10.1039/C5AN00933B

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