Issue 20, 2015

Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots

Abstract

Liquid extraction surface analysis (LESA) is a surface sampling technique that allows electrospray mass spectrometry analysis of a wide range of analytes directly from biological substrates. Here, we present LESA mass spectrometry coupled with high field asymmetric waveform ion mobility spectrometry (FAIMS) for the analysis of dried blood spots on filter paper. Incorporation of FAIMS in the workflow enables gas-phase separation of lipid and protein molecular classes, enabling analysis of both haemoglobin and a range of lipids (phosphatidylcholine or phosphatidylethanolamine, and sphingomyelin species) from a single extraction sample. The work has implications for multiplexed clinical assays of multiple analytes.

Graphical abstract: Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots

Supplementary files

Article information

Article type
Paper
Submitted
08 May 2015
Accepted
15 Jul 2015
First published
15 Jul 2015
This article is Open Access
Creative Commons BY license

Analyst, 2015,140, 6879-6885

Author version available

Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots

R. L. Griffiths, A. Dexter, A. J. Creese and H. J. Cooper, Analyst, 2015, 140, 6879 DOI: 10.1039/C5AN00933B

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