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Issue 5, 2015
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Oxidative damage in DNA bases revealed by UV resonant Raman spectroscopy

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Abstract

We report on the use of the UV Raman technique to monitor the oxidative damage of deoxynucleotide triphosphates (dATP, dGTP, dCTP and dTTP) and DNA (plasmid vector) solutions. Nucleotide and DNA aqueous solutions were exposed to hydrogen peroxide (H2O2) and iron containing carbon nanotubes (CNTs) to produce Fenton's reaction and induce oxidative damage. UV Raman spectroscopy is shown to be maximally efficient to reveal changes in the nitrogenous bases during the oxidative mechanisms occurring on these molecules. The analysis of Raman spectra, supported by numerical computations, revealed that the Fenton's reaction causes an oxidation of the nitrogenous bases in dATP, dGTP and dCTP solutions leading to the production of 2-hydroxyadenine, 8-hydroxyguanine and 5-hydroxycytosine. No thymine change was revealed in the dTTP solution under the same conditions. Compared to single nucleotide solutions, plasmid DNA oxidation has resulted in more radical damage that causes the breaking of the adenine and guanine aromatic rings. Our study demonstrates the advantage of using UV Raman spectroscopy for rapidly monitoring the oxidation changes in DNA aqueous solutions that can be assigned to specific nitrogenous bases.

Graphical abstract: Oxidative damage in DNA bases revealed by UV resonant Raman spectroscopy

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Article information


Submitted
23 Dec 2014
Accepted
12 Jan 2015
First published
12 Jan 2015

Analyst, 2015,140, 1477-1485
Article type
Paper
Author version available

Oxidative damage in DNA bases revealed by UV resonant Raman spectroscopy

F. D'Amico, F. Cammisuli, R. Addobbati, C. Rizzardi, A. Gessini, C. Masciovecchio, B. Rossi and L. Pascolo, Analyst, 2015, 140, 1477
DOI: 10.1039/C4AN02364A

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