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Issue 5, 2015
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Flow-through polymerase chain reaction inside a seamless 3D helical microreactor fabricated utilizing a silicone tube and a paraffin mold

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Abstract

We introduce a new strategy for fabricating a seamless three-dimensional (3D) helical microreactor utilizing a silicone tube and a paraffin mold. With this method, various shapes and sizes of 3D helical microreactors were fabricated, and a complicated and laborious photolithographic process, or 3D printing, was eliminated. With dramatically enhanced portability at a significantly reduced fabrication cost, such a device can be considered to be the simplest microreactor, developed to date, for performing the flow-through polymerase chain reaction (PCR).

Graphical abstract: Flow-through polymerase chain reaction inside a seamless 3D helical microreactor fabricated utilizing a silicone tube and a paraffin mold

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Article information


Submitted
12 Sep 2014
Accepted
12 Dec 2014
First published
15 Dec 2014

Analyst, 2015,140, 1416-1420
Article type
Communication

Flow-through polymerase chain reaction inside a seamless 3D helical microreactor fabricated utilizing a silicone tube and a paraffin mold

W. Wu, K. T. L. Trinh and N. Y. Lee, Analyst, 2015, 140, 1416
DOI: 10.1039/C4AN01675K

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