Jump to main content
Jump to site search

Issue 1, 2015
Previous Article Next Article

Cooperative adsorption behavior of phosphopeptides on TiO2 leads to biased enrichment, detection and quantification

Author affiliations

Abstract

The adsorption behavior of phosphopeptides onto TiO2 surfaces was studied using the quartz crystal microbalance with dissipation monitoring (QCM-D) as the main experimental technique. The main focus is the characterization of the emergence of positive cooperativity under conditions where the peptides have a positively charged C-term. It is shown that when carrying no net charge, small water-soluble peptides as a rule develop positive cooperativity. The impact of the adsorption mechanism on the outcome of TiO2 based enrichment methods was investigated with the help of matrix assisted laser desorption-ionization mass spectrometry (MALDI-MS). The data presented illustrate how the phosphopeptide profile in the enriched material may deviate from that in the native sample, as cooperative phosphopeptides are overrepresented in the former. Furthermore, commonly employed washing and elution solutions may facilitate preferential release of certain peptides, leading to further bias in the recovered sample. Taken together, the results of the present study demonstrate that thorough understanding of the mechanisms behind the adsorption of phosphopeptides on the enrichment material is necessary in order to develop reliable qualitative and quantitative methods for phosphoproteomics.

Graphical abstract: Cooperative adsorption behavior of phosphopeptides on TiO2 leads to biased enrichment, detection and quantification

Back to tab navigation

Supplementary files

Publication details

The article was received on 27 Aug 2014, accepted on 14 Oct 2014 and first published on 15 Oct 2014


Article type: Paper
DOI: 10.1039/C4AN01580K
Author version
available:
Download author version (PDF)
Analyst, 2015,140, 303-312

  •   Request permissions

    Cooperative adsorption behavior of phosphopeptides on TiO2 leads to biased enrichment, detection and quantification

    A. I. K. Eriksson, K. Edwards and V. Agmo Hernández, Analyst, 2015, 140, 303
    DOI: 10.1039/C4AN01580K

Search articles by author

Spotlight

Advertisements