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Issue 11, 2014
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Discrimination between bacterial phenotypes using glyco-nanoparticles and the impact of polymer coating on detection readouts

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Abstract

The identification and treatment of bacterial infections remains a major healthcare challenge, especially to ensure appropriate application of a limited spectrum of antibiotics. Here we describe a system capable of discriminating between different strains of Escherichia coli using multivalent, carbohydrate-functionalised, gold nanoparticles based on their different expression levels of the FimH adhesin. Upon binding of the glycosylated nanoparticles to FimH positive bacteria, the nanoparticles' optical properties change enabling the identification of bacteria strain. Comparison between direct conjugation, or via a linker, of the carbohydrate to the nanoparticle revealed significant effects on the performance of the detection system. Using a poly(ethylene glycol) spacer increased the stability, and specificity, of the glycosylated nanoparticles but also reduced aggregation upon bacterial binding. This prevented the well-known red-blue gold colour change, meaning spectrophometric, rather than optical, assessment methods were required. Using this method, FimH positive bacteria could be detected at approximately 1.5 × 107 colony forming units per mL.

Graphical abstract: Discrimination between bacterial phenotypes using glyco-nanoparticles and the impact of polymer coating on detection readouts

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Supplementary files

Article information


Submitted
20 Dec 2013
Accepted
23 Jan 2014
First published
24 Jan 2014

J. Mater. Chem. B, 2014,2, 1490-1498
Article type
Paper
Author version available

Discrimination between bacterial phenotypes using glyco-nanoparticles and the impact of polymer coating on detection readouts

S. Richards, E. Fullam, G. S. Besra and M. I. Gibson, J. Mater. Chem. B, 2014, 2, 1490
DOI: 10.1039/C3TB21821J

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