Issue 18, 2014

Evaluation of the role of polyelectrolyte deposition conditions in growth factor release

Abstract

Polyelectrolyte multilayer coatings were prepared from solutions of poly(methacrylic acid) and poly-L-histidine. Bone morphogenetic protein 2 (BMP-2) was adsorbed onto the surface of anodized titanium and polyelectrolyte multilayer coatings were built up on top of the BMP-2. The effect of deposition conditions on coating properties and preosteoblast response was measured by comparing coatings prepared under natural conditions to those prepared from solutions at pH = 6.0 and solutions containing 0.1 M NaCl. High levels of BMP-2 release were achieved, with coatings prepared from pH = 6.0 solutions releasing 86 ng cm−2 and coatings prepared from solutions containing 0.1 M NaCl releasing 114 ng cm−2 over 25 days. Enhanced preosteoblast differentiation was observed on coatings prepared from modified solutions; however, this increased differentiation was apparent for BMP-2-eluting and control coatings. Additionally, a positive relationship between surface roughness and differentiation was observed, which may account for increased differentiation for systems that do not release BMP-2.

Graphical abstract: Evaluation of the role of polyelectrolyte deposition conditions in growth factor release

Supplementary files

Article information

Article type
Paper
Submitted
10 Dec 2013
Accepted
24 Feb 2014
First published
24 Feb 2014

J. Mater. Chem. B, 2014,2, 2680-2687

Evaluation of the role of polyelectrolyte deposition conditions in growth factor release

A. M. Peterson, C. Pilz-Allen, H. Möhwald and D. G. Shchukin, J. Mater. Chem. B, 2014, 2, 2680 DOI: 10.1039/C3TB21757D

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