Issue 14, 2014

Perfect nematic order in confined monolayers of spindle-shaped cells

Abstract

Elongated, weakly interacting, apolar, fibroblast cells (mouse fibroblasts NIH-3T3) cultured at confluence align together, forming large domains (correlation length ∼ 500 μm) where they are perfectly ordered. We study the emergence of this mesoscopic nematic order by quantifying the ordering dynamics in a two-dimensional tissue. Cells are initially very motile and the monolayer is characterized by anomalous density fluctuations, a signature of far-from-equilibrium systems. As the cell density increases because of proliferation, the cells align with each other forming these large oriented domains while, at the same time, the cellular movements and the density fluctuations freeze. Topological defects that are characteristic of nematic phases remain trapped at long times thereby preventing the development of infinite domains. When confined within adhesive stripes of given widths (from 30 μm to 1.5 mm) cells spontaneously align with the domain edges. This orientation then propagates toward the pattern center. For widths smaller than the orientation correlation length, cells perfectly align in the direction of the stripe. Experiments performed in cross-shaped patterns show that in the situation of two competing populations, both the number of cells and the degree of alignment impact the final orientation.

Graphical abstract: Perfect nematic order in confined monolayers of spindle-shaped cells

Supplementary files

Article information

Article type
Paper
Submitted
03 Sep 2013
Accepted
03 Oct 2013
First published
04 Oct 2013

Soft Matter, 2014,10, 2346-2353

Perfect nematic order in confined monolayers of spindle-shaped cells

G. Duclos, S. Garcia, H. G. Yevick and P. Silberzan, Soft Matter, 2014, 10, 2346 DOI: 10.1039/C3SM52323C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements