Jump to main content
Jump to site search

Issue 14, 2014

Intracellular stresses in patterned cell assemblies

Author affiliations

Abstract

Confining cells on adhesive patterns allows performing robust, weakly dispersed, statistical analysis. A priori, adhesive patterns could be efficient tools to analyze intracellular cell stress fields, in particular when patterns are used to force the geometry of the cytoskeleton. This tool could then be very helpful in deciphering the relationship between the internal architecture of the cells and the mechanical, intracellular stresses. However, the quantification of the intracellular stresses is still something delicate to perform. Here we first propose a new, very simple and original method to quantify the intracellular stresses, which directly relates the strain the cells impose on the extracellular matrix to the intracellular stress field. This method is used to analyze how confinement influences the intracellular stress field. As a result, we show that the more confined the cells are, the more stressed they will be. The influence of the geometry of the adhesive patterns on the stress patterns is also discussed.

Graphical abstract: Intracellular stresses in patterned cell assemblies

Associated articles

Supplementary files

Article information


Submitted
02 Sep 2013
Accepted
12 Dec 2013
First published
17 Dec 2013

Soft Matter, 2014,10, 2414-2423
Article type
Paper

Intracellular stresses in patterned cell assemblies

M. Moussus, C. D. Loughian, D. Fuard, M. Courçon, D. Gulino-Debrac, H. Delanoë-Ayari and A. Nicolas, Soft Matter, 2014, 10, 2414 DOI: 10.1039/C3SM52318G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements