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Issue 5, 2014
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Metal-catalyzed uncaging of DNA-binding agents in living cells

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Abstract

Attachment of alloc protecting groups to the amidine units of fluorogenic DNA-binding bisbenzamidines or to the amino groups of ethidium bromide leads to a significant reduction of their DNA affinity. More importantly, the active DNA-binding species can be readily regenerated by treatment with ruthenium catalysts in aqueous conditions, even in cell cultures. The catalytic chemical uncaging can be easily monitored by fluorescence microscopy, because the protected products display both different emission properties and cell distribution to the parent compounds.

Graphical abstract: Metal-catalyzed uncaging of DNA-binding agents in living cells

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Supplementary files

Article information


Submitted
03 Dec 2013
Accepted
30 Jan 2014
First published
31 Jan 2014

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2014,5, 1901-1907
Article type
Edge Article
Author version available

Metal-catalyzed uncaging of DNA-binding agents in living cells

M. I. Sánchez, C. Penas, M. E. Vázquez and J. L. Mascareñas, Chem. Sci., 2014, 5, 1901
DOI: 10.1039/C3SC53317D

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