Issue 109, 2014

Synthesis of ethyl (R)-4-cyano-3-hydroxybutyrate in high concentration using a novel halohydrin dehalogenase HHDH-PL from Parvibaculum lavamentivorans DS-1

Abstract

We identified and characterized a novel halohydrin dehalogenase HHDH-PL from Parvibaculum lavamentivorans DS-1. Study of substrate specificity indicated that HHDH-PL possessed a high activity toward ethyl (S)-4-chloro-3-hydroxybutanoate ((S)-CHBE). After optimizations of the pH and temperature, whole cell catalysis of HHDH-PL was applied to the synthesis of ethyl (R)-4-cyano-3-hydroxybutyrate (HN) at 200 g L−1 of (S)-CHBE, which gave 95% conversion and 85% yield in 14 h.

Graphical abstract: Synthesis of ethyl (R)-4-cyano-3-hydroxybutyrate in high concentration using a novel halohydrin dehalogenase HHDH-PL from Parvibaculum lavamentivorans DS-1

Supplementary files

Article information

Article type
Communication
Submitted
02 Nov 2014
Accepted
17 Nov 2014
First published
19 Nov 2014

RSC Adv., 2014,4, 64027-64031

Author version available

Synthesis of ethyl (R)-4-cyano-3-hydroxybutyrate in high concentration using a novel halohydrin dehalogenase HHDH-PL from Parvibaculum lavamentivorans DS-1

N. Wan, Z. Liu, K. Huang, Z. Shen, F. Xue, Y. Zheng and Y. Shen, RSC Adv., 2014, 4, 64027 DOI: 10.1039/C4RA13646B

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