Issue 110, 2014

Suppressing Pseudomonas aeruginosa adhesion via non-fouling polymer brushes

Abstract

In the current study, well-defined polymer brushes are shown as an effective surface modification to resist biofilm formation from opportunistic pathogens. Poly[oligo(ethylene glycol)methyl ether methacrylate] (poly(MeOEGMA)) and poly[N-(2-hydroxypropyl)methacrylamide] (poly(HPMA)) brushes were grown by surface initiated atom transfer radical polymerization (SI-ATRP) and subsequently characterized by Fourier-transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS) and dynamic water contact angle measurements. Their remarkable resistance to protein fouling after long term contact with biological media was evidenced by surface plasmon resonance spectroscopy. Challenging these brushes with an environmental strain of Pseudomonas aeruginosa in mineral media as well as a casein–soja–pepton–agar (CASO) medium resulted in no biofilm formation, while a decrease of the biofilm formation by 70% (poly(HPMA)) and 90% (poly(MeOEGMA)) was observed when the medium was rich in nutrients and proteins (fetal bovine serum). In contrast to the antibiotic sensitive strains, biofilm formation was observed using an antibiotic multi-resistant P. aeruginosa strain on both brushes. Protein fouling was fully prevented on both types of brushes, which might challenge the proposed mechanism of biofilm formation mediated by a pre-formed conditioning film of proteins. The resistance to biofilm formation and the possibility to precisely control their growth and functionalities makes these brushes ((poly(HPMA) and (poly(MeOEGMA)) promising candidates for surface modification of various biomaterials as well as platforms for basic studies into the mechanisms of bacteria fouling.

Graphical abstract: Suppressing Pseudomonas aeruginosa adhesion via non-fouling polymer brushes

Supplementary files

Article information

Article type
Paper
Submitted
18 Oct 2014
Accepted
17 Nov 2014
First published
19 Nov 2014

RSC Adv., 2014,4, 64781-64790

Author version available

Suppressing Pseudomonas aeruginosa adhesion via non-fouling polymer brushes

C. Rodriguez-Emmenegger, A. Decker, F. Surman, C. M. Preuss, Z. Sedláková, N. Zydziak, C. Barner-Kowollik, T. Schwartz and L. Barner, RSC Adv., 2014, 4, 64781 DOI: 10.1039/C4RA12663G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements