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Issue 97, 2014
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Polyurea dendrimer for efficient cytosolic siRNA delivery

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Abstract

The design of small interfering RNA (siRNA) delivery materials showing efficacy in vivo is at the forefront of nanotherapeutics research. Polyurea (PURE-type) dendrimers are ‘smart’ biocompatible 3D polymers that unveil a dynamic and elegant back-folding mechanism involving hydrogen bonding between primary amines at the surface and tertiary amines and ureas at the core. Similarly, to a biological proton pump, they are able to automatically and reversibly transform their conformation in response to pH stimulus. Here, we show that PURE-G4 is a useful gene silencing platform showing no cellular toxicity. As a proof of concept we investigated the PURE-G4-siRNA dendriplex, which was shown to be an attractive platform with high transfection efficacy. The simplicity associated with the complexation of siRNA with polyurea dendrimers makes them a powerful tool for efficient cytosolic siRNA delivery.

Graphical abstract: Polyurea dendrimer for efficient cytosolic siRNA delivery

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Supplementary files

Article information


Submitted
01 Sep 2014
Accepted
07 Oct 2014
First published
07 Oct 2014

RSC Adv., 2014,4, 54872-54878
Article type
Paper
Author version available

Polyurea dendrimer for efficient cytosolic siRNA delivery

R. B. Restani, J. Conde, P. V. Baptista, M. T. Cidade, A. M. Bragança, J. Morgado, I. J. Correia, A. Aguiar-Ricardo and V. D. B. Bonifácio, RSC Adv., 2014, 4, 54872
DOI: 10.1039/C4RA09603G

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