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Issue 97, 2014
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Polyurea dendrimer for efficient cytosolic siRNA delivery

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Abstract

The design of small interfering RNA (siRNA) delivery materials showing efficacy in vivo is at the forefront of nanotherapeutics research. Polyurea (PURE-type) dendrimers are ‘smart’ biocompatible 3D polymers that unveil a dynamic and elegant back-folding mechanism involving hydrogen bonding between primary amines at the surface and tertiary amines and ureas at the core. Similarly, to a biological proton pump, they are able to automatically and reversibly transform their conformation in response to pH stimulus. Here, we show that PURE-G4 is a useful gene silencing platform showing no cellular toxicity. As a proof of concept we investigated the PURE-G4-siRNA dendriplex, which was shown to be an attractive platform with high transfection efficacy. The simplicity associated with the complexation of siRNA with polyurea dendrimers makes them a powerful tool for efficient cytosolic siRNA delivery.

Graphical abstract: Polyurea dendrimer for efficient cytosolic siRNA delivery

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Publication details

The article was received on 01 Sep 2014, accepted on 07 Oct 2014 and first published on 07 Oct 2014


Article type: Paper
DOI: 10.1039/C4RA09603G
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Citation: RSC Adv., 2014,4, 54872-54878

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    Polyurea dendrimer for efficient cytosolic siRNA delivery

    R. B. Restani, J. Conde, P. V. Baptista, M. T. Cidade, A. M. Bragança, J. Morgado, I. J. Correia, A. Aguiar-Ricardo and V. D. B. Bonifácio, RSC Adv., 2014, 4, 54872
    DOI: 10.1039/C4RA09603G

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