Electrodeposition of gold nanoparticles on a pectin scaffold and its electrocatalytic application in the selective determination of dopamine

Abstract

A simple electrochemical deposition strategy is proposed for the preparation of gold nanoparticles (Au NPs) at the electrode surface using biopolymer pectin as stabilizing agent. The formation of the nanoparticles was confirmed by scanning electron microscopy (SEM), UV-visible spectroscopy and X-ray diffraction (XRD) studies. A pectin-stabilized, gold nanoparticle film-modified glassy carbon electrode (pectin–Au NP/GCE) was prepared, which exhibited excellent electrocatalytic ability towards oxidation of dopamine (DA). At the pectin–Au NP/GCE, the redox couple corresponding to the redox reaction of DA was observed at the formal potential of 0.20 V with highly enhanced peak currents. A thin layer of nafion coating was applied on the pectin–Au NP composite to improve its selectivity. Two linear ranges of detection were found: (1) 20 nM to 0.9 μM with a limit of detection (LOD) of 6.1 nM, (2) 0.9 μM to 1 mM with a LOD of 0.64 μM. The fabricated sensor selectively detects DA even in the presence of high concentrations of interferents. Moreover, practical feasibility of the sensor was addressed in pharmaceutical samples, which presented appreciable recovery results. The main advantages of the sensor are its very simple and green fabrication approach, roughed and stable structure, and fast and highly reproducible detection of dopamine.