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Issue 93, 2014
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Direct monitoring of the interaction between ROS and cerium dioxide nanoparticles in living cells

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Abstract

A method for the direct monitoring of the interaction of reactive oxygen species (ROS) with cerium dioxide nanoparticles (CDN) in living cells is proposed based on the use of a complex of calcein and CDN. The CDN–calcein complex penetrates easily into a living cell and is decomposed readily by endogenous or exogenous ROS, releasing brightly fluorescent calcein, which can be observed using conventional fluorescent microscopy. This complex is less cytotoxic than individual cerium dioxide nanoparticles and provides effective protection from oxidative stress induced by the action of hydrogen peroxide, treatment with latex beads, or infection by the vesicular stomatitis virus.

Graphical abstract: Direct monitoring of the interaction between ROS and cerium dioxide nanoparticles in living cells

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Supplementary files

Article information


Submitted
07 Aug 2014
Accepted
22 Sep 2014
First published
22 Sep 2014

RSC Adv., 2014,4, 51703-51710
Article type
Paper

Direct monitoring of the interaction between ROS and cerium dioxide nanoparticles in living cells

N. M. Zholobak, A. B. Shcherbakov, E. O. Vitukova, A. V. Yegorova, Y. V. Scripinets, I. I. Leonenko, A. Ye. Baranchikov, V. P. Antonovich and V. K. Ivanov, RSC Adv., 2014, 4, 51703
DOI: 10.1039/C4RA08292C

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