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Abstract | Cited by

An adapted biocompatible version of the Kinugasa reaction, the copper-catalysed alkyne-nitrone cycloaddition followed by rearrangement (CuANCR), was developed for live-cell labelling. CuANCR labelling was demonstrated for both mammalian and bacterial cells. A method for metabolic incorporation of the nitrone group is also described.

Graphical abstract: Copper-catalysed cycloaddition reactions of nitrones and alkynes for bioorthogonal labelling of living cells

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