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Issue 86, 2014
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Dual enzyme responsive and targeted nanocapsules for intracellular delivery of anticancer agents

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Abstract

We report the fabrication of dual enzyme responsive hollow nanocapsules which can be targeted to deliver anticancer agents specifically inside cancer cells. The enzyme responsive elements, integrated in the nanocapsule walls, undergo degradation in the presence of either trypsin or hyaluronidase leading to the release of encapsulated drug molecules. These nanocapsules, which were crosslinked and functionalised with folic acid, showed minimal drug leakage when kept in pH 7.4 PBS buffer, but released the drug molecules at a rapid rate in the presence of either one of the triggering enzymes. Studies on cellular interactions of these nanocapsules revealed that doxorubicin loaded nanocapsules were taken up by cervical cancer cells via folic acid receptor medicated endocytosis. Interestingly the nanocapsules were able to disintegrate inside the cancer cells and release doxorubicin which then migrated into the nucleus to induce cell death. This study indicates that these nanocapsules fabricated from biopolymers can serve as an excellent platform for targeted intracellular drug delivery to cancer cells.

Graphical abstract: Dual enzyme responsive and targeted nanocapsules for intracellular delivery of anticancer agents

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Article information


Submitted
30 Jul 2014
Accepted
17 Sep 2014
First published
17 Sep 2014

RSC Adv., 2014,4, 45961-45968
Article type
Paper
Author version available

Dual enzyme responsive and targeted nanocapsules for intracellular delivery of anticancer agents

K. Radhakrishnan, J. Tripathy, D. P. Gnanadhas, D. Chakravortty and A. M. Raichur, RSC Adv., 2014, 4, 45961
DOI: 10.1039/C4RA07815B

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