A highly sensitive resonance Rayleigh scattering assay for detection of Hg(ii) using immunonanogold as probe†
Abstract
The monoclonal antibody (mAb) against Hg2+ was produced by the hybridoma technique, and was labeled using nanogold (NG) to obtain an immunonanogold (ING) probe. The immunoreaction takes place to form an ING–Hg2+ immunocomplex that caused the resonance Rayleigh scattering peak at 580 nm to decrease, with a detection limit of 1.1 nmol L−1 Hg2+.